Date on Master's Thesis/Doctoral Dissertation
Oral Health and Rehabilitation
Darling, Douglas S.
BACKGROUND: Xerostomia causes oral infections, tooth decay, and hindered digestion. Crucial to oral homeostasis is the salivary proteome. How these proteins are trafficked through secretory cells into secretory pathways is unknown. Abundant in the saliva is Parotid Secretory Protein (PSP), secreted through the regulated secretory pathway. HYPOTHESIS: The addition of XBP-1 and Mist1, known for differentiation marker regulation, to ParC10 cells will improve stimulated secretion of PSP. METHODS: Fusion clones were transiently or stably transfected into ParC10 cells. Media from non-stimulated and stimulated cells was harvested and luciferase activity was assayed. RESULTS: Stimulated secretion of cypridina-PSP was not significantly higher than non-stimulated secretion; suggesting cypridina-PSP did not enter the regulated secretory pathway. Stably transfected ParC10 showed similar results. CONCLUSION: Cypridina-PSP, XBP-1, and Mist1 did not show stimulated secretion and thus, no cell differentiation was observed. The ParC10 cells lack a regulated secretory pathway.
Pyle, Ian S. 1986-, "XBP1 and MIST1 are not sufficient to induce the regulated secretory pathway in parotid cell lines." (2013). Electronic Theses and Dissertations. Paper 1170.