Date on Master's Thesis/Doctoral Dissertation
Committee Co-Chair (if applicable)
Zamborini, Francis Patrick
Salicylic acid; Mass spectrometry
The biosynthetic pathway leading to the production of anacardic acids (AnAcs) in glandular trichomes on the surface of geranium plants remains unclear. Anacardic acids are salicylic acid derivatives known to possess various medicinal benefits. Gene silencing techniques, such as RNA interference, could be useful in determining specific enzymes involved in the synthesis of AnAcs. However, it is first necessary to develop methodologies to image AnAcs. In this project, both fluorescence (FL) and mass spectral imaging (MSI) were tested. Our results indicate that cellular imaging of AnAcs, via FL, is not suitable because the spectral ranges of absorption and emission of AnAcs fall within the same range of common cellular components. Furthermore, FL cannot distinguish among various AnAcs. Fortunately, and with the use of a new and stable matrix, bis(p-nitrophenly) amine, in-situ imaging of AnAcs using MALDI-MSI has been achieved for the first time.
Wessel, Bryan Edward 1989-, "Analysis of anacardic acid on the surface of Pelargonium × hortorum using matrix assisted laser desorptoin ionization mass spectrometry imaging." (2015). Electronic Theses and Dissertations. Paper 2146.