Date on Master's Thesis/Doctoral Dissertation
Anatomical Sciences and Neurobiology
Petruska, Jeffrey Charles
CD2AP; Collateral sprouting; Characterization
Cellular signal transduction; Sensory neurons
Collateral sprouting (CS) occurs when uninjured axons respond to denervated tissue surrounding the axon by growing branches of the axon to reinnervate the denervated zone. This process is signaled by factors released by the denervated tissue. Nerve growth factor (NGF) has been shown to be required and sufficient for the initiation and maintenance of CS. Previous work showed CD2 associated protein (CD2AP) mRNA increased following a spared dermatome procedure to model collateral sprouting. CD2AP links the cytoskeleton to tyrosine kinase receptors such as TrkA (the NGF receptor), where it is involved in the cellular response to growth factor signaling. Here we provided a novel characterization of CD2AP protein in naive sensory neurons and assessed whether it was regulated during CS. CD2AP immunostaining was observed in a variety of staining intensities and populations of cells but was not regulated in collateral sprouting as assessed by immunohistochemistry.
Couch, Sarah Elizabeth Claypool 1987-, "Expression pattern of CD2AP in the intact and collaterally sprouting nervous system." (2011). Electronic Theses and Dissertations. Paper 279.