Date on Master's Thesis/Doctoral Dissertation


Document Type

Master's Thesis

Degree Name



Bioinformatics and Biostatistics

Committee Chair

Wu, Dongfeng

Author's Keywords

MicroRNA microarray; Normalization techniques; Repeated measures; Aging mice models


RNA--Analysis; Probability measures; Genetics--Research--Methodology


The present experimental designs have unique challenges in the analysis of microRNA expression intensity levels. In this project, various Normalization techniques were discussed with different statistical techniques and their application in an analysis performed at the Genes Centre of aging on mice models. The project involved repeated measures over time, and the intra-subject correlation caused by the repeated measurements should be incorporated into the analysis, resulting in additional significant miRNAs. Collection of multiple samples from the same individuals over time from an experiment may have issues with the commonly used methodologies-these issues are discussed here. Mixed model approach is used here to see the difference between different normalized data with the raw data with repeated measurements.