Date on Master's Thesis/Doctoral Dissertation


Document Type

Master's Thesis

Degree Name




Degree Program

Biology, MS

Committee Chair

Running, Mark

Committee Co-Chair (if applicable)

Matoba, Nobuyuki

Committee Member

Matoba, Nobuyuki

Committee Member

Perlin, Mike

Committee Member

Schultz, David


Lignin peroxidase is an enzyme secreted by white-rot fungi Phanerochaete chrysosporium. Our laboratory is attempting to produce several lignin degrading enzymes in our protein expression system that utilizes the moss, Physcomitrella patens. During our experiments we discovered that this particular species of fungus produces altered splicing variants that are not represented in the previously reported reference sequence. Despite several attempts to produce a positive transformant containing a desired transgene encoding; lignin peroxidase, production of the proper construct eluded our efforts, possibly due to toxic effects associated with lignin peroxidase expression. Our laboratory has created a stepping stone for other laboratories to integrate into future research relating to the expression of fungal proteins in a moss heterologous protein expression system.

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Biology Commons