Date on Master's Thesis/Doctoral Dissertation


Document Type

Doctoral Dissertation

Degree Name

Ph. D.



Degree Program

Chemistry, PhD

Committee Chair

Zhang, Xiang

Committee Member

Zamborini, Francis

Committee Member

Li, Ying

Committee Member

Kirpich, Irina

Author's Keywords

mass spectrometry; metabolomics; lipidomics; GC-MS; LC-MS


Metabolomics and lipidomics studies are important to understand living organisms at the molecular level. In this dissertation, I conducted various untargeted and targeted metabolomics and lipidomics studies using mass spectrometry based analytical platforms. Firstly, I investigated the androgen depletion effects on fecal metabolites in a mouse model of systemic lupus erythematosus and metabolite biomarkers for colorectal cancer using two-dimensional gas and liquid chromatography-mass spectrometry. Secondly, I investigated the relationship between gut microbiota-mediated resistance and susceptibility to malaria and the production of short-chain fatty acids using gas chromatography-mass spectrometry. Thirdly, I developed a high-throughput liquid chromatography-mass spectrometry method to profile long-chain fatty acids and oxylipins. And finally, I developed a comprehensive two-dimensional liquid chromatography-mass spectrometry method for untargeted lipidomics.