Date on Master's Thesis/Doctoral Dissertation
8-2021
Document Type
Doctoral Dissertation
Degree Name
Ph. D.
Department
Chemistry
Degree Program
Chemistry, PhD
Committee Chair
Zhang, Xiang
Committee Co-Chair (if applicable)
Zamborini, Francis
Committee Member
Zamborini, Francis
Committee Member
Li, Ying
Committee Member
Kirpich, Irina
Author's Keywords
mass spectrometry; metabolomics; lipidomics; GC-MS; LC-MS
Abstract
Metabolomics and lipidomics studies are important to understand living organisms at the molecular level. In this dissertation, I conducted various untargeted and targeted metabolomics and lipidomics studies using mass spectrometry based analytical platforms. Firstly, I investigated the androgen depletion effects on fecal metabolites in a mouse model of systemic lupus erythematosus and metabolite biomarkers for colorectal cancer using two-dimensional gas and liquid chromatography-mass spectrometry. Secondly, I investigated the relationship between gut microbiota-mediated resistance and susceptibility to malaria and the production of short-chain fatty acids using gas chromatography-mass spectrometry. Thirdly, I developed a high-throughput liquid chromatography-mass spectrometry method to profile long-chain fatty acids and oxylipins. And finally, I developed a comprehensive two-dimensional liquid chromatography-mass spectrometry method for untargeted lipidomics.
Recommended Citation
Yuan, Fang, "Mass spectrometry based metabolomics and Lipidomics." (2021). Electronic Theses and Dissertations. Paper 3707.
https://doi.org/10.18297/etd/3707
