Date on Master's Thesis/Doctoral Dissertation


Document Type

Doctoral Dissertation

Degree Name

Ph. D.



Degree Program

Chemistry, PhD

Committee Chair

Zhang, Xiang

Committee Co-Chair (if applicable)

Zamborini, Francis

Committee Member

Zamborini, Francis

Committee Member

Li, Ying

Committee Member

Kirpich, Irina

Author's Keywords

mass spectrometry; metabolomics; lipidomics; GC-MS; LC-MS


Metabolomics and lipidomics studies are important to understand living organisms at the molecular level. In this dissertation, I conducted various untargeted and targeted metabolomics and lipidomics studies using mass spectrometry based analytical platforms. Firstly, I investigated the androgen depletion effects on fecal metabolites in a mouse model of systemic lupus erythematosus and metabolite biomarkers for colorectal cancer using two-dimensional gas and liquid chromatography-mass spectrometry. Secondly, I investigated the relationship between gut microbiota-mediated resistance and susceptibility to malaria and the production of short-chain fatty acids using gas chromatography-mass spectrometry. Thirdly, I developed a high-throughput liquid chromatography-mass spectrometry method to profile long-chain fatty acids and oxylipins. And finally, I developed a comprehensive two-dimensional liquid chromatography-mass spectrometry method for untargeted lipidomics.