Date on Master's Thesis/Doctoral Dissertation

11-2024

Document Type

Master's Thesis

Degree Name

M. Eng.

Department

Chemical Engineering

Committee Chair

Gupta, Gautam

Committee Co-Chair (if applicable)

Yaddanapudi, Kavitha

Committee Member

Yaddanapudi, Kavitha

Committee Member

Willing, Gerold

Author's Keywords

Biopreservation; Sol gel encapsulation; room temperature storage; BioCaRGOS

Abstract

Currently, cryovials on dry ice or liquid nitrogen vapor transportation vessels are primary ways utilized to transport mammalian cells from one location to another. Due to the safety concerns of dry ice and liquid nitrogen, associated costly shipping charges, and limitations of proper packaging and labeling of materials, these conventional shipping techniques are unacceptable. It is urgent to develop a safe and environmentally friendly protocol to preserve and ship the biological samples from one place to another. In this research, a method was developed for an ecofriendly and safe protocol to preserve cancer cells. The cell viability of cell line A549 is shown to be preserved 0.5% sol-gel at room temperature for over a week is shown to have viability of nearly 78% displaying the potential for sol-gel to be a room temperature storage method. these research findings indicated that our 0.5% sol-gel based approach was feasible for long-distance live cell transportation, and preservation at room temperature and in semi-solid gel state.


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