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Protein Prenylation is the addition of lipids to select proteins that play a key role during the development of plants. There are three enzymes that play a role in protein prenylation: protein farnesyltransferase (PFT), protein geranylgeranyl-transferase-I (PGGT), and Rab geranylgeranyltransferase (Rab-GGT). However, there is a protein called PPAL that has a similar alpha subunit of PFT, PGGT, and RAB-GGT but its biochemical function is unknown. Physconmitrella patens, a type of moss, was chosen to explore the role of PpPPAL in the development process. There are two copies of PPAL found in moss. PPAL1 and PPAL2. To study the role of these genes, a partial knockdown line was created where one or both alpha and beta subunit genes was reduced. An artificial microRNA was created to target the PpPPAL1 and PpPPAL2 genes and was introduced into the moss via a plasmid. Once the microRNA was inserted into the moss, the moss was grown in DMSO/ beta-estradiol medium to start the suppression of gene expression. The results showed that the knockout of PpPPAL 1 and PpPPAL 2 inhibits the growth and propagation of physconmitrella patens. They also resulted in a few gametophore contents. The result indicates that PPAL plays an important role in the developmental process in P.patents. The role of PPAL in protein prenylation of physconmitrella patens give insights into how prenylation works in humans since defects in prenylation can lead to health problems
Protein Prenylation, PpPPAL, Physconmitrella patens
Molecular Genetics | Plant Biology | Plant Breeding and Genetics
Perez-Martinez, Susana; Chen, Christine; and Running, Mark P., "The Study of PPAL and its Role in the Development of Physcomitrella patens" (2021). Undergraduate Arts and Research Showcase. 60.