Date on Master's Thesis/Doctoral Dissertation
12-2005
Document Type
Doctoral Dissertation
Degree Name
Ph. D.
Department
Physiology and Biophysics
Committee Chair
Ping, Peipei
Author's Keywords
Cardiac; Proteasomes; Protein degradation; Phosphorylation
Subject
Proteomics; Homeostasis
Abstract
20S proteasome, essential component of protein degradation mechanism, is important to maintain homeostasis. Its malfunctions have been associated with several pathological conditions. This study presents an extensive study of murine cardiac 20S proteasome. Using biochemical methods, 20S proteasome have been purified to 95%. Proteomic study identified all 20S proteasome subunits. Endogenous phosphorylation was also documented. Furthermore, several associating kinases and phosphatase were identified. They regulated its activities. In PKCå over-expression mice, 20S proteasome expression level was up-regulated, but its peptidase activities did not increase. áB crystallin were recruited to PKCå subproteome in the transgenic mice, which also associated with 20S proteasome. This association was enhanced in the transgenic mice and has been reported to inhibit 20S proteasome activities. It suggested áB crystallin play a role in cardiac 20s proteasome regulation.
Recommended Citation
Zong, Chenggong, "Explore the murine cardiac 20S proteasomes : molecular composition and regulation." (2005). Electronic Theses and Dissertations. Paper 1654.
https://doi.org/10.18297/etd/1654
