Date on Master's Thesis/Doctoral Dissertation
8-2010
Document Type
Master's Thesis
Degree Name
M.S.
Department
Oral Biology
Committee Chair
Darling, Douglas S.
Author's Keywords
Epigenetics; Methylation; Salivary; Gland; De-differentiation; Parotid
Subject
Epigenesis
Abstract
Background: When rat salivary gland cells are cultured in vitro, they dedifferentiate within 24 h. Hypothesis: Growth factors will prevent de-differentiation of primary cultures of parotid gland cells, and they will induce differentiation of ParC5 cells. DNA methylation drives de-differentiation and the loss of expression in cultured parotid cells. Methods: Rat parotid glands were collected and cultured in media using different combinations of growth factors. DNA was evaluated for methylation at various time points. Results: No media tested prevented the de-differentiation of parotid cells, and none induced differentiation of ParC5 cells. Limited variation in methylation of CpG sites was seen. Conclusion: Growth factors do not prevent de-differentiation in primary cell culture, nor do they activate differentiation in ParC5 cells. Global methylation of Mist1 does not cause de-differentiation; evidence supports that methylation of PSP increases over time.
Recommended Citation
Currie, Andrew Scott 1983-, "De-differentiation of parotid acinar cells in vitro." (2010). Electronic Theses and Dissertations. Paper 303.
https://doi.org/10.18297/etd/303
