Plasma cysteine/cystine and glutathione/glutathione disulfide redox potentials in HIV and COPD patients.

Authors

Walter H. Watson, University of Louisville
Jeffrey D. Ritzenthaler, University of Louisville
Paula Peyrani, University of Louisville Infectious DiseasesFollow
Timothy L. Wiemken, Division of Infectious Diseases, Department of Medicine, University of Louisville School of Medicine, Louisville, KYFollow
Stephen P. Furmanek, University of Louisville, Division of Infectious DiseasesFollow
Andrea Reyes-Vega, University of LouisvilleFollow
Tom J. Burke, University of Louisville
Yuxuan Zheng, University of LouisvilleFollow
Julio A. Ramirez, University of LouisvilleFollow
Jesse Roman, University of Louisville School of Medicine Division of PulmonaryFollow

Document Type

Article

Publication Date

11-1-2019

Department

Medicine

Abstract

Chronic obstructive pulmonary disease (COPD) is prevalent in patients infected with HIV. The purpose of this study was to test the hypothesis that systemic oxidation correlates with loss of lung function in subjects with COPD, and that HIV infection can contribute to creating such an environment. Subjects were recruited at the University of Louisville in the following groups: HIV-infected (n = 36), COPD (n = 32), HIV and COPD (n = 28), and uninfected controls with normal lung function (n = 34). HIV infection was assessed by viral load and CD4 cell counts. Pulmonary function was determined by spirometry, and plasma was collected for measurement of cysteine (Cys), cystine (CySS), glutathione (GSH) and GSH disulfide (GSSG) by HPLC followed by estimation of redox potentials (Eh) using the Nernst equation. Results showed that patients with COPD had more oxidized plasma Eh Cys/CySS than patients with normal lung function, but plasma Eh GSH/GSSG was unaltered. In addition, there was a correlation between the extent of plasma Eh Cys/CySS oxidation and loss of lung function, and this correlation remained even after correcting for age, sex, race and body mass index. HIV infection per se was not associated with increased oxidation of plasma Eh Cys/CySS, but plasma Eh Cys/CySS was more oxidized in patients with lower CD4-positve T cell counts. In patients with both HIV infection and COPD, there was a significant correlation between CD4 cell counts and lung function. Thus, systemic oxidation correlated with decreased lung function in subjects with COPD and decreased CD4 counts in subjects infected with HIV. Thus, factors contributing to plasma Eh Cys/CySS may represent novel mechanisms underlying the increased prevalence of COPD in people living with HIV.

Graphical abstract

Original Publication Information

Received 16 April 2019; Received in revised form 10 June 2019; Accepted 28 July 2019

Author: Walter H. Watson,Jeffrey D. Ritzenthaler,Paula Peyrani,Timothy L. Wiemken,Stephen Furmanek,Andrea M. Reyes Vega,Tom J. Burke,Yuxuan Zheng,Julio A. Ramirez,Jesse Roman Publication: Free Radical Biology and Medicine Publisher: Elsevier

1 November 2019

Available online 29 July 2019 0891-5849/ © 2019 Published by Elsevier Inc.

ThinkIR Citation

Watson, Walter H.; Ritzenthaler, Jeffrey D.; Peyrani, Paula; Wiemken, Timothy L.; Furmanek, Stephen P.; Reyes-Vega, Andrea; Burke, Tom J.; Zheng, Yuxuan; Ramirez, Julio A.; and Roman, Jesse, "Plasma cysteine/cystine and glutathione/glutathione disulfide redox potentials in HIV and COPD patients." (2019). Faculty Scholarship. 958.
https://ir.library.louisville.edu/faculty/958

DOI

doi.org/10.1016/j.freeradbiomed.2019.07.031

ORCID

0000-0003-1633-6097