Date on Master's Thesis/Doctoral Dissertation
Oral Biology, MS
Committee Co-Chair (if applicable)
Background: Aggregatibacter actinomycetemcomitans (Aa) possesses a two-component system, QseBC, which is key in biofilm formation/virulence and is activated in the presence of iron-catecholamine complexes. Aa does not synthesize enterobactin, a catechol-based siderophore, yet possesses the machinery for recognition and uptake. We hypothesize that Aa is able to acquire iron by catecholamine mediation through the enterobactin receptor/transporter. Methods: By insertional mutation, we attempted to delete the enterobactin permease from the genome using suicide vector pJT1. A growth curve of truncated mutant ΔfepA was also conducted to observe growth in the presence of iron and catecholamines. Statistical significance was determined by ANOVA (p
Johnson, Taylor, "Function of the enterobactin operon of A. actinomycetemcomitans in the presence of catecholmines and iron." (2017). Electronic Theses and Dissertations. Paper 2706.