Date on Master's Thesis/Doctoral Dissertation


Document Type

Master's Thesis

Degree Name



Oral Biology

Degree Program

Oral Biology, MS

Committee Chair

Demuth, Donald

Committee Co-Chair (if applicable)

Sandell, Lisa

Committee Member

Sandell, Lisa

Committee Member

Lamont, Rich


Background: Aggregatibacter actinomycetemcomitans (Aa) possesses a two-component system, QseBC, which is key in biofilm formation/virulence and is activated in the presence of iron-catecholamine complexes. Aa does not synthesize enterobactin, a catechol-based siderophore, yet possesses the machinery for recognition and uptake. We hypothesize that Aa is able to acquire iron by catecholamine mediation through the enterobactin receptor/transporter. Methods: By insertional mutation, we attempted to delete the enterobactin permease from the genome using suicide vector pJT1. A growth curve of truncated mutant ΔfepA was also conducted to observe growth in the presence of iron and catecholamines. Statistical significance was determined by ANOVA (p