Date on Master's Thesis/Doctoral Dissertation
Committee Co-Chair (if applicable)
anthropology; immunology; bioarchaeology
Individuals who mount a strong inflammatory response may produce a shift in the systemic levels of inflammatory mediators, which may lead to a potential hyper-inflammatory phenotype (HIP). Systemic inflammation can increase severity in local inflammatory processes detected in bone lesions. This thesis investigated in vitro if human immune cells pre-treated with inflammatory inducers would affect the inflammatory response against Porphyromonas gingivalis or Staphylococcus aureus (both associated with osteological lesions). We exposed human peripheral blood mononuclear cells to bacterial lysates, or pro-inflammatory cytokines. Sequentially, we exposed the same culture to either P. gingivalis or S. aureus. The final expression of TNFα and IFNγ was measured by ELISA. Our results showed that early exposure to the inflammatory inducers increased the expression of inflammatory cytokines. These findings could be useful in osteological analyses when considering how systemic inflammation may affect local inflammatory responses, and how this could be influenced by a HIP.
Duncanson, Megan Elizabeth, "Experimental immunology and the potential for osteopathological reconstructions : pursuing an experimental foundation for the skeletal inflammatory index." (2019). Electronic Theses and Dissertations. Paper 3179.