Functionalizing nanoparticles with CafA protein to target BAR peptide for oral delivery applications.
Date on Master's Thesis/Doctoral Dissertation
Oral Biology, MS
Committee Co-Chair (if applicable)
Porphyromonas gingivalis; streptococci gordonii; peptide delivery; nanoparticles; delivery vehicle; surface-modified nanoparticles
Background: Porphyromonas gingivalis adherence to Streptococcus gordonii may be important for P. gingivalis colonization in the oral cavity. Nanoparticles encapsulating synthetic peptide BAR (BAR-NPs) inhibit P. gingivalis adherence more potently than free BAR. However, BAR-NPs would exhibit low retention in an open flow environment. Hypothesis: Targeting BAR-NPs to the streptococcal surface using CafA protein will enhance their efficacy. Methods: CafA-modified NPs encapsulating BAR were synthesized using double emulsion approach. Surface binding and retention, and release kinetics of BAR from CafA-modified NPs was assessed. Functional inhibition assays were performed using dual a species biofilm. Results: CafA-modified NPs demonstrated specificity of adhesion, remained bound to S. gordonii surfaces and released inhibitory concentrations of BAR for over 8hr. CafA-modified NPs inhibited P. gingivalis adherence to S. gordonii potently for over 8hr. Conclusions: CafA-modified NPs represent a delivery vehicle that targets BAR to preferred niches of P. gingivalis in the oral cavity.
Desai, Hetal, "Functionalizing nanoparticles with CafA protein to target BAR peptide for oral delivery applications." (2020). Electronic Theses and Dissertations. Paper 3460.