Date on Master's Thesis/Doctoral Dissertation

1-2021

Document Type

Master's Thesis

Degree Name

M.S.

Department

Pharmacology and Toxicology

Degree Program

Pharmacology and Toxicology, MS

Committee Chair

Siskind, Leah

Committee Co-Chair (if applicable)

Beverly, Levi

Committee Member

Beverly, Levi

Committee Member

Clark, Geoff

Committee Member

Donninger, Howard

Committee Member

Bates, Paula

Committee Member

Mitchell, Robert

Author's Keywords

SRC; NSCLC; cell-derived matrix

Abstract

Lung cancer is responsible for the most cancer deaths between males and females, with a 6% five-year survival rate. Cancer metastasis is one of the leading causes of lung cancer lethality. Our lab has developed a method to culture cells on cell-derived matrix (CDM) and study cell-ECM interactions. This study utilized multiple cell migration methods to investigate the migration of NSCLC cells and IMR90 lung fibroblasts, both together and independently, on 2D plastic and 3D CDM. Our results showed that inhibiting Src, a significant influencer of cell migration and member of integrin activation, blocked cancer cell migration on 2D and 3D CDM, regardless of interactions with fibroblasts or not. However, when IMR90 lung fibroblasts were treated with a Src inhibitor, migration decreased on 2D but not on 3D. These results suggest that cancer cells migrate in a Src-dependent manner on 3D CDM, but IMR90 lung fibroblasts do not.

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