Date on Master's Thesis/Doctoral Dissertation

12-2022

Document Type

Doctoral Dissertation

Degree Name

Ph. D.

Department

Interdisciplinary and Graduate Studies

Degree Program

Interdisciplinary Studies (Individualized Degree), PhD

Committee Chair

Giridharan, Guruprasad

Committee Co-Chair (if applicable)

Mohamed, Tamer

Committee Member

Roussel, Thomas

Committee Member

Williams, Stuart

Committee Member

El-Baz, Ayman

Author's Keywords

Heart; cardiac tissue slices; cardio-oncology; reverse cardio-oncology; tissue culture; cardiotoxicity

Abstract

28% of drug withdrawal from the market are due to unforeseen disruptions in cardiomyocyte contractility and electrophysiology. The most commonly used platforms for drug testing are in vivo animal models and in vitro cell culture models. While both have been of paramount importance for the discovery and detection of many cardiotoxicities and mechanisms of action, they lack the ability to model an intact human myocardium. This work aims to establish cardiac tissue slices, which are 300-micron thin tissue sections taken from the left ventricular myocardium, as an alternative platform for cardio-oncology studies, specifically cardiotoxicity testing. Additionally, this work aims to develop a coculture bioreactor system that can be used in reverse cardio-oncology related studies to investigate the potential crosstalk between cardiovascular disease and tumorigenesis.

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