Date on Master's Thesis/Doctoral Dissertation


Document Type

Master's Thesis

Degree Name



Mechanical Engineering

Committee Chair

Williams, Stuart J., 1981-

Author's Keywords

Impedance sensing; Endothelial cells; Shear stress


Vascular endothelium; Blood-vessels--Pathophysiology; Vascular endothelial cells


Understanding the endothelium at the cellular level could further knowledge of the cardiovascular system as a whole and could therefore lead to advances in the prevention and treatment of cardiovascular disease. Electric cell-substrate impedance sensing is an in vitro technique that can be used for observing the behavior of endothelial cells in real-time using a fluid flow environment to simulate the circulatory system. This study examined the effect of fluid shear stress on human umbilical vein endothelial cells using electrochemical impedance spectroscopy (impedance sensing). Circuit models were fit to empirical data to measure cell layer resistance and capacitance changes, and to determine if data trends follow those of previously published findings. Information derived from transendothelial electrical resistance measurements, about changes in cell layer permeability when subjected to varying shear stress conditions within an orbiting circular well, was used to draw conclusions aided by microscopic images of the cells.