Date on Master's Thesis/Doctoral Dissertation
8-2021
Document Type
Master's Thesis
Degree Name
M.S.
Department
Oral Biology
Degree Program
Oral Biology, MS
Committee Chair
Steinbach-Rankins, Jill
Committee Co-Chair (if applicable)
Demuth, Donald
Committee Member
Scott, David
Committee Member
Pisano, Michele
Author's Keywords
3D-bioprinting; probiotics
Abstract
Background: Porphyromonas gingivalis adheres to and invades gingival epithelial cells, resulting in decreased cell viability. Previous studies have indicated that probiotics are effective against dental pathogens; however, few approaches provide sustained-delivery of active agents in the oral cavity. Hypothesis: Probiotics will limit P. gingivalis effects on Telomerase Immortalized Gingival Keratinocyte (TIGK) cells and 3D-printed scaffolds will prolong probiotic release. Methods: Lactobacillus acidophilus, Lactobacillus reuteri, and Bifidobacterium bifidum were assessed with adhesion and antibiotic protection assays to limit P. gingivalis effects on TIGKs. Scaffolds were printed with a select probiotic and evaluated for release kinetics. Results: Free L.a., L.r., and B.b. administration improved TIGK viability by reducing P. gingivalis adhesion. Additionally, probiotic-containing scaffolds were successfully printed, demonstrating high viability and sustained-release of probiotics over two weeks. Conclusion: Probiotics effectively limit P. gingivalis adhesion to TIGKs, suggesting that 3D-bioprinted probiotic-containing scaffolds may be a promising delivery system for mitigating P. gingivalis colonization.
Recommended Citation
Patel, Jhanvi, "Development of 3D-bioprinted scaffolds for oral probiotic application." (2021). Electronic Theses and Dissertations. Paper 4249.
Retrieved from https://ir.library.louisville.edu/etd/4249
