Date on Master's Thesis/Doctoral Dissertation

8-2021

Document Type

Master's Thesis

Degree Name

M.S.

Department

Oral Biology

Degree Program

Oral Biology, MS

Committee Chair

Steinbach-Rankins, Jill

Committee Co-Chair (if applicable)

Demuth, Donald

Committee Member

Scott, David

Committee Member

Pisano, Michele

Author's Keywords

3D-bioprinting; probiotics

Abstract

Background: Porphyromonas gingivalis adheres to and invades gingival epithelial cells, resulting in decreased cell viability. Previous studies have indicated that probiotics are effective against dental pathogens; however, few approaches provide sustained-delivery of active agents in the oral cavity. Hypothesis: Probiotics will limit P. gingivalis effects on Telomerase Immortalized Gingival Keratinocyte (TIGK) cells and 3D-printed scaffolds will prolong probiotic release. Methods: Lactobacillus acidophilus, Lactobacillus reuteri, and Bifidobacterium bifidum were assessed with adhesion and antibiotic protection assays to limit P. gingivalis effects on TIGKs. Scaffolds were printed with a select probiotic and evaluated for release kinetics. Results: Free L.a., L.r., and B.b. administration improved TIGK viability by reducing P. gingivalis adhesion. Additionally, probiotic-containing scaffolds were successfully printed, demonstrating high viability and sustained-release of probiotics over two weeks. Conclusion: Probiotics effectively limit P. gingivalis adhesion to TIGKs, suggesting that 3D-bioprinted probiotic-containing scaffolds may be a promising delivery system for mitigating P. gingivalis colonization.

Download

Share

COinS