Date on Master's Thesis/Doctoral Dissertation
8-2016
Document Type
Master's Thesis
Degree Name
M.S.
Department
Pharmacology and Toxicology
Degree Program
Pharmacology and Toxicology, MS
Committee Chair
Siskind, Leah
Committee Member
Beverly, Levi
Committee Member
Arteel, Gavin
Committee Member
Lederer, Eleanor
Committee Member
Clark, Geoff
Author's Keywords
nephrotoxicity; cisplatin; fibrosis; kidney; AKI; CKD
Abstract
Cisplatin is a nephrotoxic chemotherapeutic that causes acute kidney injury (AKI) in 30% of patients. Although recovery can occur after one episode of cisplatin-induced AKI, studies have indicated multiple episodes may lead to the development of chronic kidney disease (CKD), an irreversible disease with no current treatments. The standard mouse model of cisplatin-induced AKI consists of one, high dose of cisplatin (> 20 mg/kg) that is lethal to the animal three days later. This model doesn’t accurately reflect the repeated dosing regimen patients receive, and doesn’t allow for long-term outcome studies of pathologies associated with CKD. We have developed a repeated dosing model of cisplatin (7mg/kg once a week for four weeks). This model allows for the long-term survival of mice, and the associated pathology is fibrosis-the hallmark of CKD. Thus, data indicate that the repeated dosing model can be used to study AKI to CKD progression.
Recommended Citation
Sharp, Cierra N., "Developing a more clinically-relevant mouse model of cisplatin-induced nephrotoxicity." (2016). Electronic Theses and Dissertations. Paper 2524.
https://doi.org/10.18297/etd/2524
