Date on Master's Thesis/Doctoral Dissertation
8-2019
Document Type
Master's Thesis
Degree Name
M.S.
Department
Biology
Degree Program
Biology, MS
Committee Chair
Running, Mark
Committee Co-Chair (if applicable)
Matoba, Nobuyuki
Committee Member
Matoba, Nobuyuki
Committee Member
Perlin, Mike
Committee Member
Schultz, David
Abstract
Lignin peroxidase is an enzyme secreted by white-rot fungi Phanerochaete chrysosporium. Our laboratory is attempting to produce several lignin degrading enzymes in our protein expression system that utilizes the moss, Physcomitrella patens. During our experiments we discovered that this particular species of fungus produces altered splicing variants that are not represented in the previously reported reference sequence. Despite several attempts to produce a positive transformant containing a desired transgene encoding; lignin peroxidase, production of the proper construct eluded our efforts, possibly due to toxic effects associated with lignin peroxidase expression. Our laboratory has created a stepping stone for other laboratories to integrate into future research relating to the expression of fungal proteins in a moss heterologous protein expression system.
Recommended Citation
Rozsa, Jesse Lee, "Transforming moss P. patens with lignin peroxidase through heterologous protein expression." (2019). Electronic Theses and Dissertations. Paper 3270.
https://doi.org/10.18297/etd/3270
