Date on Master's Thesis/Doctoral Dissertation
8-2021
Document Type
Doctoral Dissertation
Degree Name
Ph. D.
Department
Chemistry
Degree Program
Chemistry, PhD
Committee Chair
Zhang, Xiang
Committee Member
Zamborini, Francis
Committee Member
Li, Ying
Committee Member
Kirpich, Irina
Author's Keywords
mass spectrometry; metabolomics; lipidomics; GC-MS; LC-MS
Abstract
Metabolomics and lipidomics studies are important to understand living organisms at the molecular level. In this dissertation, I conducted various untargeted and targeted metabolomics and lipidomics studies using mass spectrometry based analytical platforms. Firstly, I investigated the androgen depletion effects on fecal metabolites in a mouse model of systemic lupus erythematosus and metabolite biomarkers for colorectal cancer using two-dimensional gas and liquid chromatography-mass spectrometry. Secondly, I investigated the relationship between gut microbiota-mediated resistance and susceptibility to malaria and the production of short-chain fatty acids using gas chromatography-mass spectrometry. Thirdly, I developed a high-throughput liquid chromatography-mass spectrometry method to profile long-chain fatty acids and oxylipins. And finally, I developed a comprehensive two-dimensional liquid chromatography-mass spectrometry method for untargeted lipidomics.
Recommended Citation
Yuan, Fang, "Mass spectrometry based metabolomics and Lipidomics." (2021). Electronic Theses and Dissertations. Paper 3707.
https://doi.org/10.18297/etd/3707
