Date on Master's Thesis/Doctoral Dissertation
8-2023
Document Type
Master's Thesis
Degree Name
M.S.
Department
Pharmacology and Toxicology
Degree Program
Pharmacology and Toxicology, MS
Committee Chair
Mohamed, Tamer M.
Committee Co-Chair (if applicable)
Siskind, Leah J.
Committee Member
Siskind, Leah J.
Committee Member
Jones, Steven P.
Committee Member
Clark, Geoffrey J.
Author's Keywords
Cardiac regeneration; cardiomyocyte; heart failure; myocardial infarctionEpigenetic
Abstract
The limited regenerative capacity of adult cardiomyocytes (CMs) is manifested in prevalent morbidity and mortality associated with ischemic heart disease. We previously demonstrated that a combination of four cell cycle factors (4F) promotes efficient cell division in differentiated CMs. The temporal transcriptional reprogramming in the 4F-transduced CMs indicates that epigenetic modifications mediate the cell cycle progression. Through small molecule screening, we evaluated the epigenetic impact on CM’s response in the 4F-transduced CMs. We identified the histone demethylase 5B (KDM5B), which through in vitro and ex vivo genetic knockdown and overexpression studies, exhibited a modulative effect on CM’s cell cycle plasticity along with transcriptional alteration in the cell cycle-related genes. Whereas KDM5B depletion impaired CM’s replicative capacity, its overexpression induced and augmented CM’s proliferation in a context-dependent manner. Our data indicate that KDM5B is essential for CM’s regeneration and cell cycle activation and is a potential target for myocardial repair.
Recommended Citation
Hammouri, Dana, "KDM5B as a potential epigenetic regulator of cardiomyocyte regeneration and cell cycle activation." (2023). Electronic Theses and Dissertations. Paper 4148.
https://doi.org/10.18297/etd/4148