Date on Master's Thesis/Doctoral Dissertation
12-2023
Document Type
Master's Thesis
Degree Name
M.S.
Department
Oral Biology
Degree Program
Oral Biology, MS
Committee Chair
Potempa, Jan
Committee Co-Chair (if applicable)
Bagaitkar, Juhi
Committee Member
Bagaitkar, Juhi
Committee Member
Graham, James
Committee Member
Uriarte, Silvia
Author's Keywords
Porphyromonas gingivalis; Gingipains; protein purification; protease purification
Abstract
Porphyromonas gingivalis is a keystone oral pathogen in the progression of periodontal disease and has been implicated in the etiology of many other systemic human diseases. P. gingivalis produces primary virulence factors termed the gingipains, a group of cysteine proteases. There is a high demand for high purity gingipains for downstream in vitro studies where P.g. is implicated, both oral and systemic. Gingipain production candidate mutant P.g. strains were generated to isolate each gingipain for large scale purification and the purified gingipains characterized. Two mutants were determined not to be viable for large scale purification of gingipains, each for different reasons, which may give some insight into novel mechanisms of adaption in P. gingivalis.
Recommended Citation
Fuchs, Andrew E., "Genetically engineering Gingipains for high yield large scale purification." (2023). Electronic Theses and Dissertations. Paper 4193.
https://doi.org/10.18297/etd/4193
