Date on Master's Thesis/Doctoral Dissertation
Oral Biology, MS
Porphyromonas gingivalis; Gingipains; protein purification; protease purification
Porphyromonas gingivalis is a keystone oral pathogen in the progression of periodontal disease and has been implicated in the etiology of many other systemic human diseases. P. gingivalis produces primary virulence factors termed the gingipains, a group of cysteine proteases. There is a high demand for high purity gingipains for downstream in vitro studies where P.g. is implicated, both oral and systemic. Gingipain production candidate mutant P.g. strains were generated to isolate each gingipain for large scale purification and the purified gingipains characterized. Two mutants were determined not to be viable for large scale purification of gingipains, each for different reasons, which may give some insight into novel mechanisms of adaption in P. gingivalis.
Fuchs, Andrew E., "Genetically engineering Gingipains for high yield large scale purification." (2023). Electronic Theses and Dissertations. Paper 4193.
Retrieved from https://ir.library.louisville.edu/etd/4193