Submission Type
Poster
Abstract
The mountain pine beetle (MPB, Dendroctonus ponderosae) is a pest species found in western Norh America that attacks and kills Pinus host trees. Previous studies have identified genetic divergence between populations and variation in life-history traits (body size and development time) that are likely important in local adaptation. To understand the underlying genetic architecture of MPB adaptation and divergence, we performed an F1 intercross between two distinct populations (Utah & Arizona) and performed a QTL analysis. We analyzed double digest RAD sequencing data from 181 F2 individuals. Our final dataset consists of 986 SNPs and we were able to successfully map all 12 linkage groups and characterize recombination across the genome. We identified a significant QTL peak for development time on the X chromosome. No significant QTL was detected for body size. Lastly, we compared our linkage map to an existing map created using MPB populations from Canada. We found the linkage maps were largely similar, suggesting no major genome structure differences or changes in general patterns of recombination across different populations.
Included in
QTL Analysis of Body Size and Development Time in Dendroctonus ponderosae
The mountain pine beetle (MPB, Dendroctonus ponderosae) is a pest species found in western Norh America that attacks and kills Pinus host trees. Previous studies have identified genetic divergence between populations and variation in life-history traits (body size and development time) that are likely important in local adaptation. To understand the underlying genetic architecture of MPB adaptation and divergence, we performed an F1 intercross between two distinct populations (Utah & Arizona) and performed a QTL analysis. We analyzed double digest RAD sequencing data from 181 F2 individuals. Our final dataset consists of 986 SNPs and we were able to successfully map all 12 linkage groups and characterize recombination across the genome. We identified a significant QTL peak for development time on the X chromosome. No significant QTL was detected for body size. Lastly, we compared our linkage map to an existing map created using MPB populations from Canada. We found the linkage maps were largely similar, suggesting no major genome structure differences or changes in general patterns of recombination across different populations.
Comments
Ryan R. Bracewell, Indiana University Bloomington
Barbara J. Bentz, USDA Forest Service