Date on Master's Thesis
7-2025
Document Type
Master's Thesis
Degree Name
M. Eng.
Department
Bioengineering
Committee Chair
Dr. Frieboes, Hermann
Committee Member
Dr. Chen, Joseph
Committee Member
Dr. Sathitsuksanoh, Noppadon
Abstract
Catheter-associated urinary tract infections (CAUTIs) are a major healthcare burden with current prevention strategies offering limited success. This study presents a novel, self-coating biomaterial approach using a silicone-based bioink embedded with thermally sensitive probiotic strains (L. crispatus, L. rhamnosus, and L. reuteri). Catheter segments were fabricated via bio-injection molding using 3D-printed CAD molds. These Lactobacilli, selected for their probiotic and antimicrobial potential, serve as a non- antibiotic strategy to reduce infection risk. These segments were evaluated for mechanical strength (Shore A hardness), thermal stability (TGA/DSC), porosity and bacterial presence (SEM), biofilm formation (crystal-violet assay), as well as mass retention in artificial urine. Results showed favorable physical and thermal properties and effective Lactobacilli colonization, supporting the potential of this self-coating probiotic catheter system as a preventative measure against CAUTI.
Recommended Citation
Maners, Caden, "Characterization of Lactobacilli-containing Catheter Segments Developed by Using Selective Laser Sintered Mold." (2025). Master of Engineering Theses. Paper 5.
Retrieved from https://ir.library.louisville.edu/speed_meng/5
Included in
Biological Engineering Commons, Biomaterials Commons, Female Urogenital Diseases and Pregnancy Complications Commons, Male Urogenital Diseases Commons, Molecular, Cellular, and Tissue Engineering Commons
