The Cardinal Edge


Arts and Research Showcase 2023


This article investigates the effect of osmotic stress on a drosophila cell line called Kc167.

The embryonic-derived fly (Drosophila melanogaster) cell line, Kc-167, was employed as a model for water-stress sensitivity in Arthropods. Like mammalian cells, cells derived from the fruit fly contain the same basic set of membranous components found in all eukaryotic cells. A series of experiments were conducted to characterize the mitochondrial repones of Kc167 cells to water stress. Precisely, the oxygen flux in a sealed respirometer chamber containing Kc167 cells was measured under hyperosmotic and control conditions. Mitochondrial uncouplers were used in some experiments for intact and chemically permeabilized cells to gain detailed information on mitochondrial integrity in response to increased solute concentration. Mitochondria are the primary ATP producer in the cell and consume oxygen in a process termed oxidative phosphorylation. Therefore, oxygen consumption rates can be used to assess the impact of water-limited states on cellular bioenergetics. Conducted experiments were performed to measure the following:

• The basal oxygen consumption rates of Kc167 cells.

• Consumption under conditions of oxidative stress

• The oxygen consumption of chemically permeabilized cells

• The max mitochondrial uncoupling that the Kc167 could withstand.

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