The Impact of Prenatal Vape Exposure on Weanling Liver Gene Expression

Authors

Isabella R. Fieger, University of LouisvilleFollow
Cristina Isabel Pauig, University of LouisvilleFollow
Mindy Yin, University of LouisvilleFollow
Anna Lipinski, University of LouisvilleFollow
Barrett Blevins, University of LouisvilleFollow
Tyler Plake, University of LouisvilleFollow
Lucas Georges, University of LouisvilleFollow
Katelyn Chism, University of LouisvilleFollow
Isaiah Burciaga, University of LouisvilleFollow
Selma Podbicanin, University of LouisvilleFollow
Cynthia Corbitt, University of LouisvilleFollow
Rachel Neal, University of LouisvilleFollow

Program/Event

Arts and Research Showcase 2023

Abstract

The use of E-cigarettes is a fairly recent phenomenon. Vaping is seen as the “healthier” alternative to smoking cigarettes, yet we know little about the developmental toxicity of commercially available vaping products. In the current study, C57Bl/6 mice were exposed to Vuse Alto Golden Tobacco pods (5% nicotine) 4 days before mating and throughout gestation (GD19) for 1 hour/day every day. Offspring birth outcomes were measured with liver tissue collected at weaning. Gross histology and gene expression in the SIRT1-FXR pathway were examined via qPCR analysis with male and female offspring analyzed separately. No differences in gross morphology or cell area were found between the Vape and Sham group offspring (neither male or female). Female Vape offspring exhibited reduced Sirt1 gene expression when compared to Sham offspring, with no impact noted for males. Additional downstream genes (PEPCK, HK, LXR) were also analyzed with trends toward an impact of prenatal Vape exposure noted though the outcomes varied.

Recommended Citation

Fieger, Isabella R.; Pauig, Cristina Isabel; Yin, Mindy; Lipinski, Anna; Blevins, Barrett; Plake, Tyler; Georges, Lucas; Chism, Katelyn; Burciaga, Isaiah; Podbicanin, Selma; Corbitt, Cynthia; and Neal, Rachel (2021) "The Impact of Prenatal Vape Exposure on Weanling Liver Gene Expression," The Cardinal Edge: Vol. 1: Iss. 4, Article 5.
Available at: https://ir.library.louisville.edu/tce/vol1/iss4/5