Program/Event
2024 Undergraduate Arts & Research Showcase Spring 2024
Abstract
Breast cancer is the second leading cause of death amoung women after heart disease. One of the deadliest types of breast cancer, triple negative breast cancer (TNBC), is resistant to many chemotherapy drugs. Chemotherapeutics that can inhibit TNBC growth are needed to combat this form of cancer. Historically, natural compounds found in plants have been studied for their effectiveness at limiting cancer cell growth while also reducing negative side effects due to lower toxicity to healthy cells. Many chemotherapeutics have serious side effects that can result in reduced treatment compliance, often leading to the recurrence of more aggressive and recalcitrant cancer. Cumin, a culinary spice harvested from Cuminum cyminum, has exhibited anticancer bioactivities in TNBC. Our lab produced an acid-hydrolyzed (AH) extract with increased anticancer activity and employed a nanoparticle delivery system to try and enhance the delivery of cumin phytochemicals. MDA-MB-231 (TNBC) cells were exposed to inhibitory concentrations of AH-cumin extracts and AH-cumin extract nanoparticle formulations. Transcripts of p53 (a tumor suppressor involved in apoptosis) were measured using RT-qPCR and compared using the ΔΔCt method with GAPDH as a reference housekeeping gene. p53 transcripts were significantly reduced in the extract treatment, but nanoparticle formulations were not significantly different than controls.
Recommended Citation
Wheeler, Krista A.; Scott, Jared L.; and Schultz, David
(2024)
"Cumin Seed Extracts Can Affect the Expression of p53 in Triple Negative Breast Cancer,"
The Cardinal Edge: Vol. 2:
Iss.
2, Article 19.
Available at:
https://ir.library.louisville.edu/tce/vol2/iss2/19
