Program/Event
2023 Summer Research Showcase
Abstract
The use of E-cigarettes is a fairly recent phenomenon. Vaping is thought of as the “healthier” alternative to smoking cigarettes, yet we know little about the developmental toxicity of commercially available vaping products. The Barker Hypothesis (or the Developmental Basis of Adult Disease Hypothesis) proposes that developmental nutrient restriction or toxicant exposure increases the risk of diseases later in life such as cardiovascular disease, fatty liver, and metabolic diseases. In this study, adult female C57Bl/6 mice were exposed to Vuse™ Alto Golden Tobacco pods (5% nicotine) 4 days before mating and throughout gestation (GD19) for 1 hour/day each day. Offspring birth outcomes were measured with liver tissue collected at weaning. Gene expression in the FXR-SIRT1 pathway (major nutrient response pathway) was examined. Male and female offspring were analyzed separately. Female Vape offspring exhibited reduced Sirt1 gene expression in the fasted state, but males did not. Male Vape offspring exhibited a trend toward decreased FXR gene expression in the fasted state, but females did not. Additional downstream genes, such as p53, HK, LXR, and ACC, showed trends toward a significant impact of prenatal Vape exposure on gene expression, though the outcomes varied. Overall, early changes in the central biomolecular pathway are evident in both female and male Vape groups.
Recommended Citation
Yin, Mindy; Podbicanin, Selma; Fieger, Bella; Pauig, Nina; Chism, Katelyn; Georges, Lucas; Burciaga, Isaiah; Corbitt, Cynthia; and Neal, Rachel
(2024)
"The Effect of Prenatal Vape Exposure on Liver Gene Expression in Weanling Mice,"
The Cardinal Edge: Vol. 2:
Iss.
2, Article 4.
Available at:
https://ir.library.louisville.edu/tce/vol2/iss2/4
